The Effects of Age and Surface Characteristics on Proliferation and Differentiation of Human Jaw Bone Osteoblast Response to Titanium Implant

Ken-chung Chen DDS, MS, Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan
Tzer-min Lee , Institute of Oral Medicine, National Cheng Kung University Medical College, Tainan City, Taiwan
Chih-han Chang , Department of Biomedical Engineering, National Cheng Kung University, Tainan City, Taiwan
Tung-Yiu Wong DDS, Department of Oral and Maxillofacial Surgery, National Cheng Kung University Hospital, Tainan, Taiwan
Jing-Jing Fang PhD, Department of Mechanical Engineering, National Cheng Kung University, Tainan, Taiwan

The effects of age and surface characteristics on proliferation and differentiation of human jaw bone osteoblast reponse to titanium implant

Background: Osseointegration is defined as "direct structural and functional connection between living

bone and the surface of a load-bearing artificial implant", osseointegrated implant can bear functional loading.The host response and the properties of the implant surface are considered as the key factors for the success of osseointegration. However, little is known about the effects of aging on the molecular responses of bone cells, especially the jaw bone osteoblasts, to the implant materials having different surface surface teatments.Purpose: to investigate the influence of different implant surface topographies and different age on the expression of differentiation/proliferation markers on jaw bone osteoblasts of human. Material and method: Titanium disc with different surface treatments including sandblasting, sandblasting and acid etching(SLA) and microarc oxidation (MAO) were produced and surface roughness was detected by profilemeter and topography were observed by SEM. Tissue were harvested from human jaw bone and cultured to osteoblast in vitro, then seeded onto titanium disc. The growth of osteoblast of different ages from human on all surfaces were compared. Alkaline phosphatase (ALP) activity were tested to determine cell differentiation at day 3, day7, day 14 and Alarmar Blue assay were tested to determine cell proliferation at day 1, day 3, day 7, day 14.Ostepcalcin (OC) and Osteprotegerin (OPG) were measured at day 14.Statistics by Kruskal-Wallis method Results: 15 samples were collected and osteoblast from human jaw bone was successfully primary cultured. In surface effect, MAO surface is better in cell proliferation and differentiation. In age effect, no difference between different age about cell proliferation but significant difference was noted on differentiation marker (ALP and OPG). Conclusions:Appropriate surface treatment is important to the mechanism of osseointegration and age does play some roles in osseointegration.

References :

1. Hai Zhang et al. The effects of patient age on human osteoblasts' response toTi-6A1-4V implants in vitro Journal of Orthopaedic Research 2004;22: 30-38

2. Carlo Galli et al. Comparison of Human Mandibular Osteoblasts Grown on Two Commercially Available Titanium Implant Surfaces J Periodontol 2005;76(3):364-372

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