The Effect of Biomechanical Stimulation on Osteoblast Differentiation of Human Jaw Periosteum-derived Stem Cells
Materials and Methods: Human peristeal derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4 % cyclic equibiaxial tension force (0.5Hz) was applied for 2 and 8 hours on the stem cells, and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group.
Results: ALP activity and ALP mRNA expression level were higher in the strain group than those of the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group to the control group on days 7 and 14. The VEGF mRNA expression was higher in the strain group in comparison to the control group. Concentration of Alizarin Red S corresponding to calcium content was higher in the strain group than the control group.
Conclusion: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.