The Effect of Biomechanical Stimulation on Osteoblast Differentiation of Human Jaw Periosteum Derived Stem Cells

Ju-min Lee PhD, Dept. of Dental Anesthesiology, Pusan National University Dental Hospital, Yangsan, South Korea
Uk-kyu Kim DDS, PhD, Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan, South Korea

The Effect of Biomechanical Stimulation on Osteoblast Differentiation of Human Jaw Periosteum Derived Stem Cells

Ju-Min Lee, Uk-Kyu Kim

Pusan National University, School of Dentistry, Korea Republic

Purpose: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of hum periosteal derived stem cell using the newly developed bioreactor with tension force.

Methods: Human peristeal derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4 % cyclic equibiaxial tension force (0.5Hz) was applied for 2 and 8 hours on the stem cells, and cultured for 3, 7, and 14 days on the osteogenic medium.

Thereafter, biochemical changes of the osteoblasts after the biomechanical stimulation were investigated.

Results: ALP activity & ALP mRNA expression level was higher in the strain-exposed the stem cells group than the control group which was not in strain condition. The osteocalcin and osteonectin mRNA expressions were higher in the strain groups to the control groups on days 7 and 14. The VEGF m RNA expression also was higher in the strain group in comparison to the control group. Concentration of Alizarin Red S corresponding to calcium content was higher in the strain group than the control group.

Conclusion: The study suggests the cyclic biomechanical force could influence the osteoblast differentiation of periosteal derived stem cells under optimal stimulation condition and could applicable for tissue engineering.

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