The Effect of Locally Administered 15-­deoxy-­Δ12,14­Prostaglandin J©ü on Bone Regeneration in a Critical-size Segmental Defect in the Rat Fibula

Recently, there was a report that the 15-­deoxy-­Δ^12,14­-prostaglandin J₂ (15d­-PGJ₂) reduced bony destruction in the mice with metastatic breast cancer and recovered the serum levels of bone turnover markers in the ovariectomized mice.  They suggested that the 15d-PGJ₂have not only ability of reducing destruction of bone, but also potential of regeneration of bone.  On the basis of it, the purpose of this study is to evaluate the effect of 15d­-PGJ₂ on regeneration of bone.

A more than critical size defect (7mm) of fibula was created in each of 17 male Spraque-Dawley rats.  The control groups are left sides, resected and positioned with saline soaked absorbable collagen sponge(ACS, CollaHeal^TM, Bioland, Korea).  On the other hands, the experimental groups are right sides, resected and positioned with 15d­PGJ₂(Cayman Chemicals, Ann Arbor, MI, USA) (0.2mg) with ACS.  The groups were evaluated using histologic, radiologic, Micro-CT, and statistical methods following 4- (9 rats) and 8-week (8 rats) healing intervals.

 

As a result, there was no significant evidence of new bone formation in clinical observation and radiologic analysis, but that could be observed through Micro-CT, 3D images, and statistical methods in some rats.  Histologically, there were newly formed bone, mineralized matrix, and angiogenesis at the defect sites on those specimens.

Although the effect of the 15d-PGJ₂ for bony regeneration seems to be not enough, it is suspected to stimulate osteoblast and osteoclast into cycling of the metabolism of bone in the nucleus receptor unit.  Therefore, we believe that further study for the mechanism and proper concentration of 15d-PGJ₂as an effective bone graft material should be needed.

References

Kim Ki­-Rim. “15-­deoxy-­Δ^12,14­-prostaglandin J₂ inhibits bone loss of breast cancer metastasis and ovariectomy­induced osteoporosis”. Graduate school of Yonsei University, Seoul. 2012.

Koshihara Y, Kawamura M. “Prostaglandin D2 stimulates calcification of human osteoblastic cells”. Biochemical and Biophysical Research Communications. 31;159(3):1206-12. Mar, 1989.