Examination of the Process Underlying Healing of Epithelial Defects by Using Different Wound Dressings
Material: Full-thickness defects (8 mm in diameter) were created busing a biopsy punch on the backs of 7-week-old rats, and the wound areas were marked. Then, the wounded areas were covered with NEOVEIL® or TERUDERMIS® and fixed using a rat jacket. In the control rats, the wounded areas were not covered with any dressing. The rats were killed on day 4 or 7 after injury, and the wound area, neo-epithelium length, and α-SMA expression were evaluated and compared between the control, NEOVEIL®, and TERUDERMIS® groups. We measured distance and the area between markings and observed the contracture of the wound. For histopathological investigation, we measured the neo-epithelium length by hematoxylin and eosin staining and determined α-SMA expression in the wound area tissue.
Result: The epithelial defect areas in the control group on day 4 or 7 were significantly smaller than those in the NEOVEIL® and TERUDERMIS® groups. There was no significant difference in the neo-epithelium length among the control, NEOVEIL®, and TERUDERMIS® groups. The expression of α-SMA in granulation tissue on day 4 peaked in all groups. The expression of α-SMA in the control groups on day 4 or 7 after injury was greater than that in the NEOVEIL® and TERUDERMIS® groups. However, there was no significant difference between the expression of α-SMA between the NEOVEIL® and TERUDERMIS® groups.
Conclusion: In this study, the NEOVEIL® and TERUDERMIS® groups showed a suppression in contracture and lower expression of α-SMA in the wound areas. However, there was no significant difference in the neo-epithelium length among the control, NEOVEIL®, and TERUDERMIS® groups. These results suggest that the difference in the closure speed of the epithelial defect area is caused by contracture instead of the neo-epithelium length. On the basis of these results, we intend to develop a new wound-healing dressing.
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