Evaluation of Synthetic Bone Grafting Materials According to the Ratio of Hydroxyapatite and β-tricalcium Phosphate

Kim, Su-Gwan

Evaluation of synthetic bone grafting materials according to the ratio of hydroxyapatite and b-tricalcium phosphate

Su-Gwan Kim, Min-Ji Yim

Department of Oral & Maxillofacial Surgery, School of Dentistry, Chosun University, Gwangju, Korea

The purpose of this study was to compare the characteristics such as bio-compatibility and biological safety of synthetic bone grafting materials according to mixing ratios (4:6, 5:5, and 6:4) of hydroxyapatite (HA) and b-tricalcium phosphate (b-TCP)1. All of bone grafting materials were synthesized as porous spherical particles with 0.5~1.0 mm using drop-wise into liquid nitrogen to format the micropore at both internal and external surface. After synthesis of bone grafting materials according to the defined mixing ration, they were performed the characteristic analysis according to the test guide line supplied from the Ministry of Food and Drug Safety, Republic of Korea. The electronic microscopic image analysis to observe the surface and to determine the pore distribution showed that the surface of synthesized bone grafting materials were similar with travecular bone with approximately 130 mm micropore size. The pH of synthesized bone grafting materials was pH8.5~9.0. Each bone grafting materials synthesized by defined mixing ratio showed the low cytotoxicity and high cell attachment in both human normal oral keratinocyte and human osteoblast cell line MG-63. Especially, bone grafting material synthesized by mixing ratio 4:6 of HA and b-TCP showed the most bio-compatibility than that of other bone grafting materials. Furthermore, the Ca/P ration of bone grafting material synthesized by mixing ratio 4:6 of HA and b-TCP have a 1.64, that is a similar value of Ca/P of human bone and teeth2. These data are suggesting that bone grafting material synthesized by 4:6 mixing ratio of HA and b-TCP might be better biological safety, bio-compatibility, and economical cost for dental implantation.

Acknowledgement: This study was supported by the Regional Innovation Center for Dental Science & Engineering, Chosun University, Gwangju, Korea (B0008940).

References

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2. Liang C, Wang H, Yang J, Cai Y, Hu X, Yang Y, Li B, Li H, Li H, Li C, Yang X : Femtosecond laser-induced micropattern and Ca/P deposition on Ti implant surface and its acceleration on early osseointegration. ACS Appl Mater Interfaces. 5:8179-86, 2013.